Journal: Genome Biology

Article Title: Gene expression patterns during adaptation of a helminth parasite to different environmental niches

doi: 10.1186/gb-2007-8-4-r65

Figure Lengend Snippet: Some of the major transcripts from clustering analysis of the microarray data

Article Snippet: Total RNA (15 μg) for individual stages (coupled to Cy3 or Cy5) was hybridized to Combimatrix custom array microarray chips, as described in detail below (Combimatrix Corporation, Mukilteo, WA, USA).

Techniques: Microarray, Ubiquitin Proteomics, Membrane, Binding Assay

Journal: Genome Biology

Article Title: Gene expression patterns during adaptation of a helminth parasite to different environmental niches

doi: 10.1186/gb-2007-8-4-r65

Figure Lengend Snippet: Real time PCR analysis of select transcripts across four developmental stages: sporocysts (S), cercariae (C), schistosomula (L), and adult worms (A). The upward arrow indicates transcript level where fewer RT cycles are indicative of an increase in transcripts relative to a cytochrome c internal control. The microarray ratio of sporocyst to cercaria (SvC) and adult to cercaria (AvC) is designated 'fold change' and is in the same format as Figure 2. Cathepsin L numbers are from type II analysis.

Article Snippet: Total RNA (15 μg) for individual stages (coupled to Cy3 or Cy5) was hybridized to Combimatrix custom array microarray chips, as described in detail below (Combimatrix Corporation, Mukilteo, WA, USA).

Techniques: Real-time Polymerase Chain Reaction, Control, Microarray

Journal: PLoS ONE

Article Title: Comparative Genomics of Helicobacter pylori Strains of China Associated with Different Clinical Outcome

doi: 10.1371/journal.pone.0038528

Figure Lengend Snippet: General features of the sequenced H. pylori genomes selected for microarray probe design.

Article Snippet: In this study, a whole-genome CombiMatrix Custom oligonucleotide tiling microarray with 90000 probes covering six sequenced Helicobacter pylori ( H. pylori ) genomes was designed.

Techniques: Microarray

Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

Article Title: Fecal Microbial Transplants Reduce Antibiotic-resistant Genes in Patients With Recurrent Clostridium difficile Infection

doi: 10.1093/cid/ciw185

Figure Lengend Snippet: Pearson similarity index of donors over time and recurrent Clostridium difficile infection (RCDI) patients to donors. Samples were analyzed using a DNA microarray. A , Donors retained a high degree of similarity to their own samples over their donation time period. Donor 1 = 18 months; Donor 2 = 12 months; Donor 3 = 7 months ( B ) Percent similarity of the single fecal microbiota transplantation (FMT) recipient samples at those time points (1 = 1–4 weeks; 2 = 8–52 weeks post FMT [PFT]) to their respective donor. Following FMT, the similarity of the RCDI patients increased to more closely resemble the donors; **** P -value <.0001 calculated using paired t -test; *** P -value =.0005 calculated using Mann–Whitney test. C , Percent similarity of the repeated FMT recipient samples at those time points to the respective donor of the most recent FMT (PFT1: 1–2 weeks; PFT2: 3–28 weeks); * P -value <.05; ** P -value <.005 both calculated using Mann–Whitney test. Whiskers denote mean with the standard deviation. Abbreviation: BFT, before FMT.

Article Snippet: DNA was extracted and analyzed on a custom oligonucleotide-based DNA microarray containing 370 probes targeting 354 ABR genes (Lallemand Health Solutions, Montreal, Canada).

Techniques: Infection, Microarray, Transplantation Assay, MANN-WHITNEY, Standard Deviation